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Flowjo - fixing comp matrix: PE and PerCPCy5.5 leaking into FITC

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#1 Wek



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Posted 21 June 2013 - 05:28 PM

Can anyone explain how I can/should modify the comp matrix so that I can fix the compensations for my single stains? I tried modifying the numbers but I am don't think I'm doing it properly because I dont see much change in my analyses. I'm trying to correct the matrix because some of my FITC- samples are showing up as FITC+.

Attached Thumbnails

  • comp matrix.jpg
  • pe vs fitc.jpg

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