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How many samples in descriptive study

power calculation mouse microscopy

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#1 Mindbomb



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Posted 21 June 2013 - 08:46 AM

Hi all,

I've been struggling with what to write in my grant applications in the power calculations and sample size field. Most of my experiments are aimed at observing and describing in transgenic mouse by real time confocal imaging a specific cell differentiation process:

1) How something happens? Do the cells divide? Do they migrate before? etc.

2) Whether a GFP positive cell gives rise to smth else (ie. RFP positive cell) that I am interested in a particular question?

So in essence I am describing a biological process without comparing the means of different groups.

Is there a certain rule on how to present the sample size in this case? In most papers people just do these experiments n>5 times to be sure that something happens in more than one animal.

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