Celecoxib forms crystals when diluted in RPMI how do I avoid this
Posted 21 June 2013 - 02:41 AM
I need to dose my cells with celecoxib so I made a stock solution of 25mM with 100%DMSO as recommended. It was well dissolved. I further diluted it to 10mM and 1mM before my final concentration of 100uM but it forms a lot of crystal and I can see a lot of crystal in the assay. Also I think this affect the acvtivity of the drug. Can anyone advise on this please. Thanks
Posted 21 June 2013 - 04:56 AM
did you perform your initial dilutions with rpmi or dmso?
genius does what it must
i do what i get paid to do
Posted 25 June 2013 - 09:08 AM
Posted 16 October 2013 - 01:14 PM
HELLO if you sonicat your rpmi with your drug perhaps is usefull
Posted 15 January 2014 - 03:05 AM
Dont make the intermediate dilution. Add the desired amount of 25mM stock to the RPMI, mix and look for precipitate. Only add extra DMSO to the final solution if needed, no need to overcomplicate things. I think 100 uM should be OK, but you won't be able to go much higher.