Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Celecoxib forms crystals when diluted in RPMI how do I avoid this


  • Please log in to reply
4 replies to this topic

#1 Peniel

Peniel

    member

  • Active Members
  • Pip
  • 27 posts
1
Neutral

Posted 21 June 2013 - 02:41 AM

Hi all,

I need to dose my cells with celecoxib so I made a stock solution of 25mM with 100%DMSO as recommended. It was well dissolved. I further diluted it to 10mM and 1mM before my final concentration of 100uM but it forms a lot of crystal and I can see a lot of crystal in the assay. Also I think this affect the acvtivity of the drug. Can anyone advise on this please. Thanks

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,782 posts
132
Excellent

Posted 21 June 2013 - 04:56 AM

you can add a little more dmso to the rpmi (if the system can tolerate it).

did you perform your initial dilutions with rpmi or dmso?
talent does what it can
genius does what it must
i do what i get paid to do

#3 Peniel

Peniel

    member

  • Active Members
  • Pip
  • 27 posts
1
Neutral

Posted 25 June 2013 - 09:08 AM

The 10mM and 1mM were diluted in RPMI. The crystals were initially formed in the 10uM stock.

#4 antitoxin

antitoxin

    member

  • Active Members
  • Pip
  • 10 posts
1
Neutral

Posted 16 October 2013 - 01:14 PM

HELLO if you sonicat your rpmi with your drug perhaps is usefull



#5 Osibisa

Osibisa

    member

  • Active Members
  • Pip
  • 10 posts
1
Neutral

Posted 15 January 2014 - 03:05 AM

Dont make the intermediate dilution. Add the desired amount of 25mM stock to the RPMI, mix and look for precipitate. Only add extra DMSO to the final solution if needed, no need to overcomplicate things. I think 100 uM should be OK, but you won't be able to go much higher.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.