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How to choose bovine serum Albumin

BSA

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1 reply to this topic

#1 madelingirly

madelingirly

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Posted 19 June 2013 - 05:49 PM

Dear All,
I can't find our bottle of BSA.
it just disappeared, I searched every where and I asked our lab mates.
I want to order new bottle so I can continue my experiment.
I use it as blocking reagent for fluorescence immunostaining.
I can find a lot of types of BSA.
one free of IGg
one free of globulins
one normal.
I dont know which one I should order.
and if I was using normal one and I choose free globulin, it may affect my previous results??
Thank u
Madelin

#2 bob1

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Posted 20 June 2013 - 01:09 AM

We use fraction V BSA for immuno and westerns. I don't know if this has any scientific backing or if it is just the one someone ordered.





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