Posted 19 June 2013 - 10:02 AM
I base the correct value on the number that the Qubit gives me, using the protocol as described in their manual. I am wondering if anyone else has tried to use a dye based DNA quantification assay in a small volume (~25 uL) format and if similar issues were encountered. In particular, my Quant-iT values are always lower than the Qubit values (the factor varies, but it differs as much as two fold).
Thanks for the help!
PS I am new here and also new to the field
Posted 20 June 2013 - 11:31 AM
Anyway I'd try out another fluorometer...
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.