I am currently using ELISA by Salimetrics to measure for salivary cortisol. Since am a newbie, I have been using old kits which have expired since 2011. I have managed to get some data out of it but the problem is some figures are out of range and CV of wells are extremely high. I am trying to determine if the problem lies with my techniques or does the fault lies with the expired kits.
I have 2 new kits which I am going to use when I do actual runs on my samples. It is just that I have budget constraints so I unfortunately I have to learn ensure that I don't stuff during the use of my actual kits.
Can I run undiluted saliva samples or should I dilute them? I have over 60 samples.
I have attached a pdf file of the run. This run is using undiluted samples.
Any help provided is appreciated.
Edited by Fujie Derek Koh, 19 June 2013 - 09:22 AM.