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Streptavidin purification in the lab


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#1 FreddieP

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Posted 19 June 2013 - 07:09 AM

Hi, I am looking at the Biotech companies for a source of streptavidin. It's too expensive. I want to make the streptavidin myself, instead. I want to bind it to the inside of a tube. I don't have much experience with protein purification but I'm very good in the lab with most other things. Does anyone have any experience, a good protocol, sources, helpful hints for streptavidin production and purification in the lab and use for coating? Thanks, FreddieP

#2 mdfenko

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Posted 20 June 2013 - 04:33 AM

here is a wiki page, with references, which may start you on your way:

[url="http://en.wikipedia.org/wiki/Streptavidin"]streptavidin[/url]

good luck
talent does what it can
genius does what it must
i do what i get paid to do

#3 FreddieP

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Posted 20 June 2013 - 01:09 PM

Thank you for this post. I read over the page you suggested. I see there is alot of useful information there. However, I am specifically looking for protocols for working with streptavidin, specifically purification and coating. I think that this type of information must be available, somewhere, on the internet. Thank you for your help.

#4 mdfenko

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Posted 21 June 2013 - 04:37 AM

here is a [url="https://www.google.com/search?q=purification+of+streptavidin&ie=utf-8&oe=utf-8&aq=t&rls=org.mozilla:en-US:official&client=firefox-a"]google query for purification of streptavidin[/url].

(i hope you're prepared to culture streptomyces)

here is a [url="https://www.google.com/search?q=binding+streptavidin+to+6+well+plates&ie=utf-8&oe=utf-8&aq=t&rls=org.mozilla:en-US:official&client=firefox-a#client=firefox-a&hs=qaM&rls=org.mozilla:en-US%3Aofficial&sclient=psy-ab&q=how+do+i+coat+96+well+plates+with+streptavidin&oq=how+do+i+coat+96+well+plates+with+streptavidin&gs_l=serp.12...107231.125659.0.128032.48.47.1.0.0.0.140.4760.26j21.47.0.ernk_timediscounta..0.0...1.1.17.psy-ab.nzg3mOlYZwE&pbx=1&bav=on.2,or.r_qf.&bvm=bv.48293060,d.dmg&fp=be945dcd5b7bcd58&biw=1016&bih=555"]google query for coating a 96 well plate with streptavidin[/url].

(one of the pages found by the google query is in the [url="http://www.protocol-online.org/biology-forums/posts/41609.html"]bioforum archives[/url])

(it seems that standard coating techniques are used to coat a plate with streptavidin)
talent does what it can
genius does what it must
i do what i get paid to do

#5 FreddieP

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Posted 24 June 2013 - 07:32 AM

Thank you for this friendly advice. FreddieP




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