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In vitro transcription: forgot DNAse treatment!

RNA DNAse PolyA

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#1 Lilange

Lilange

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Posted 18 June 2013 - 04:18 AM

Hi all,

I forgot to do the DNAse treatment after RNA in vitro transcription, and I did directly the polyA tailing procedure. Is it necessary to do the DNAse treatment (RNA will be used for microinjection in zebrafish)? and if yes, can I do it after the polyA tailing? Or just a purification with phenol/chlo is fine?

Thank you for your help.

Lilange





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