PCR to get 10kbp product
Posted 17 June 2013 - 08:48 PM
I have to get 10kbp PCR product.
I have 2 primers, which have Tm of 58oC (F) and 60oC ®. I intend to use Pfu Taq pol to get the high accuracy.
What should I do to get success?
Thank you so much for your suggestion!
Posted 18 June 2013 - 05:58 AM
- DrLeo likes this
Posted 27 June 2013 - 02:26 AM
As you mentioned, the Elongation step you used a period of 15minutes, is it?
And the GC enhancer you mentioned is located in the target gene or somewhere else???
Posted 27 June 2013 - 06:08 AM
Thank you very much for your suggestion. It really makes sense to me.
However, have you ever tried with Q5 pol for amplifying large fragment, say > 10kbp?
Thank you again