Does someone have any experience with the boyden chambers?
I performed this assay and, in the last step, I stained the migrated cells with DAPI after fixing and permeabilizing them. I looked at them with the fluorescence and I could clearly see the blue dots of the nuclei. The problem is that I could not see cells with the bright field, so I am afraid that what I observed could be the aspecific of the membrane pores. Could this be possible? Should DAPI stain only the DNA ? Could the blue dots be migrating cells that are still stacked into the membrane pores?
Thank you very much for your help!
Transwell migration assay (Boyden chambers) and DAPI staining
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