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No tubulin detected in HL-60 lysates (???)

immunoblotting Western blotting myeloid cells

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#1 DrSnood



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Posted 14 June 2013 - 10:54 AM

Hi All,

I've been working on some experiments in HL-60 cells. Surprisingly, I tried using tubulin as a loading control for my immunoblots, and nothing was detected! (However it works fine in other cell types run on the same blot.) Actin seems to work fine, thus finding a loading control is not the issue. I'm wondering if anyone else has observed this, and what reason(s) might help to answer the case of missing tubulin.


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