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CD14+CD16++ monocyte Flow Cytometry

CD14++ CD16- monocyte monocyte CD14 CD16

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#1 chaghaleh

chaghaleh

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Posted 13 June 2013 - 10:34 AM

I do not know anything about Flow Cytometry.
My PI asked me to treat Primary monocytes and them check the phenotype of monocytes.

Have you ever done this? That is checking converting of classical monocyte (CD14++ CD16- monocyte) to non-classical monocyte (CD14+CD16++ monocyte)?

I am not sure but I think I should do these steps:

1- Buying "Human CD14+ Monocytes" from a company then:
for example from:
https://www.stemcell...-Monocytes.aspx
or
http://www.abmgood.c...ytes-T4122.html

2- Culturing in order to incease them for several passages, then:
3- Treating and Doing Flow Cytometry for CD14+ and CD16+

Is that true?
Do you have any experience? links? guideline for newbies?

Thanks





Also tagged with one or more of these keywords: CD14++ CD16- monocyte, monocyte, CD14, CD16

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