Cells adhering to walls of conical tube?
Posted 13 June 2013 - 06:07 AM
I am counting Jurkat, 3T3, HEK and CHO cells in a standard 15ml conical tube (polypropylene) or microfuge tubes. I either pipette triturate 5x minimum or invert the conical slowly 3-5x before counting on our Coulter counter to avoid settling of the cells. Either way, I can start at 7E5 cells/ml, but after an hour the concentration is around 3E5/ml. Could the cells be adhering to the conical wall even after mixing the sample?
It's quite perplexing and any suggestions would be helpful. Thanks!
- annenonor likes this
Posted 13 June 2013 - 08:49 AM
2. To your question specifically, do you count viable or total amount of cells ? Because depending on how senstitive the cells are to these conditions, viability may drop within 1 h so there will be a lesser amount of viable cells. I don't know if the Coulter counter automatically distinguishes viable from dead but I think the range of sizes to be measured can be defined in the settings.
Posted 14 June 2013 - 08:38 AM
Have you tried the more expensive low-binding tubes? I am curious to know how well they work for this situation. Thanks!