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criteria for setting dRn limit

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#1 Dora K

Dora K


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Posted 13 June 2013 - 05:16 AM


I would like to ask if anyone could help me with dRn limit

In most qualitative Real Time PCR methods the evaluation criteria are
· Ct cut-offs (max Ct shift relative to the mean Ct of the positive control)
· dRn limits ( minimum percentage of the mean dRn of the positive control)

In most cases the dRn limits is dRnpositive control x 0.2 or dRnpositive control 0.33. I was wondering how this percentage is defined. ??

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