Enzymes from Promega work in the same buffer ( B ). But my BamHI is from Fermentas and it works in unique BamHI buffer. The compositions of the buffers are not identical.
The question is: can I do double digest in Promega buffer or it is better to do a sequential digest using proper buffer for each enzyme?
Are there any differences between same enzyme but from different companies?
Thanks for help!
Edited by Angrysh, 12 June 2013 - 09:53 PM.