I'm doing a phagocytosis assay with J774 macrophage-like cell line and Cryptococcus neoformans (a yeast). The yeasts are opsonised using specific monoclonal antibody before proceeding to the phagocytosis assay. After 2 hours incubation, I noticed some extracellular yeasts remains attached outside J774 cells even after extensive washing with PBS. Are there any ways I can remove these extracellular yeasts without disrupting the macrophages?
Many thanks in advance!
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Remove extracellular cells after antibody opsonization
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