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bisulfite pcr primers


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#1 kant0008

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Posted 18 March 2004 - 10:03 PM

Hi!
I am having real trouble with bisulfite pcr at the moment, I was wondering if anyone has any suggestions on bisulfite pcr primer design (not methylation specific pcr, but pcr to amplify any bisulfite modified dna- to be sequenced later).
Thanks!

#2 sage

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Posted 19 March 2004 - 11:49 AM

Can you make your question more specific? Have you ever use the online primer design program for designing bisulfie genomic sequencing PCR primers at http://www.ucsf.edu/urogene/methprimer ?




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