I trying to transduce LSK cells with my gene of interest (cloned in MSCV-neo) for colony formation assay. Literature suggests to culture transduced cells for 7 days in selection i.e in presence of G418. I am not getting a clue whether I have to add G418 for rest part of my experiments i.e replating after 7 days.
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Selection of transduced LSK murine cellsneed sugggestion
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