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Random vs oligo primer in preamplification RT


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#1 virusman2

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Posted 09 June 2013 - 02:45 PM

I am using promega REVERRSE TRANSCRIPTIO SYSTEM to make cDNA from viral RNA. The kit provided with two, random and oligo, primers. My question is “which is better in my case(viral RNA) the random or the oligo primer?”

#2 bob1

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Posted 09 June 2013 - 04:48 PM

presumably the oligos are actually oligo dT? if this is the case - would you expect the viral transcripts to have poly A tails?




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