With one miRNA and its control (U6), I get two cDNA control contaminations: one without RTase and one without RNA template. The curves are normal and the Ct values are even better than with normal samples! The opposite controls are fine, so the only difference i.e. the primer (present in every well) cannot explain the case alone. qPCR master mix controls are fine, too.
Everything has been double-checked. Does anyone have some ideas? Thanks in advance.
Edited by GoldEpi, 07 June 2013 - 05:14 AM.