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1M IPTG left for 12-14h @ Room temp - still good?

IPTG Protein expression IPTG iptg stability stability room temp

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#1 Frager



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Posted 06 June 2013 - 05:33 PM

Hi, I accidently left my 1molar IPTG solution (in water) at room temperatur for the night. Since we are running low on IPTG as powder I really want to keep using that. Do you think it is still stable and usable for induction? Best, derFrager

Edited by Frager, 06 June 2013 - 05:33 PM.

#2 phage434



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Posted 06 June 2013 - 05:38 PM

Probably ok, in my view.

#3 labtastic



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Posted 08 June 2013 - 12:35 PM

The IPTG should be fine.

If your lab is looking for places to save money, try using autoinduction media instead of IPTG for expression. All the components are reasonably cheap (including the lactose which replaces the IPTG), and it yields significantly more dense cultures compared to standard LB/IPTG inductions (i.e. more cells and therefore more yield). It doesn't work great for all proteins, but for any reasonably well behaved protein, autoinduction is the way to go.

Also tagged with one or more of these keywords: IPTG Protein expression, IPTG, iptg stability, stability, room temp

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