After IP and reverse cross linking and DNA purification, I done a standard PCR using control primers (GAPDH), wich I aspect bands around 166 bp.
But.. This is my result of my standard PCR the samples are attached:
1- positive control (RNA pol)
2- negative control ( IgG)
3- intesest target (HA)
5- negative control of reaction (water)
I don´t found explanation for this results. probably I have product in the imput, but I guess that I don´t have nothing what I looking for. And what about the amount of fragment of the IP product? is amazing. Is that because the IP is failed?, or it could be the pcr condictions??
another question , it could be that products forms dimeric structure??. or are those inespecific products?
thanks for your point of view!!!!