Primer concentration - stupid question
Posted 04 June 2013 - 04:51 PM
In the IDT FAQ it is suggested to add 10 microliters of water per 1 nanomole to obtain 100 µM concentration. But in my calculations the final concentration is 1µM/µL. Am I wrong somwhere? How can you possibly get micromoles (10-6) from nanomoles (10-9) by dilution?
This is the link to the IDT manual http://www.idtdna.co...-concentration-
Thank for help!
Posted 04 June 2013 - 06:10 PM
Posted 04 June 2013 - 08:03 PM
Now it is clear. So molar concentration is always per liter?
Posted 05 June 2013 - 01:36 AM