Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Primer concentration - stupid question


  • Please log in to reply
3 replies to this topic

#1 Angrysh

Angrysh

    member

  • Active Members
  • Pip
  • 16 posts
2
Neutral

Posted 04 June 2013 - 04:51 PM

I've odered primers from IDT.

In the IDT FAQ it is suggested to add 10 microliters of water per 1 nanomole to obtain 100 µM concentration. But in my calculations the final concentration is 1µM/µL. Am I wrong somwhere? How can you possibly get micromoles (10-6) from nanomoles (10-9) by dilution?

This is the link to the IDT manual http://www.idtdna.co...-concentration-

Thank for help!

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,484 posts
251
Excellent

Posted 04 June 2013 - 06:10 PM

You are confusing moles with molar. 1 molar (1 M) is 1 mole per liter. The IDT instructions are 1 nanomole per 10 microliters, which is 10**-9 moles/ 10**-5 liters, or 10**-4 molar, or 100 uM.

#3 Angrysh

Angrysh

    member

  • Active Members
  • Pip
  • 16 posts
2
Neutral

Posted 04 June 2013 - 08:03 PM

Thank you!

Now it is clear. So molar concentration is always per liter?

#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,786 posts
406
Excellent

Posted 05 June 2013 - 01:36 AM

Yes, Molar concentrations are always per litre -it's the SI unit. It is a bit of an unfortunate thing that biologists (including myself) have gotten into using the "M" symbol for mol/l as it is quite confusing until you know the convention.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.