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Labeling and tracking RNA

RNA fluorescent labeling transfection

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3 replies to this topic

#1 kaveh

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Posted 04 June 2013 - 01:54 PM

Hi,

I am trying to find a way to fluorescent-label RNA molecules and track them in the cell by microscopy. My idea is to synthesize the RNA (in-vitro transcription) and transfect them into the cell. I know that you can incorporate fluorescent labeled dNTPs into the RNA (like the way one would synthesize RNA-FISH probes), but is the RNA structure/function would be unaltered? Is there any method for adding a fluorescent label to the end of the RNA, without messing with the main portion of it?

Thank you for your time

K

#2 mdfenko

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Posted 05 June 2013 - 04:27 AM

check this webpage, it may contain the information you want:

life technologies, molecular probes, rna labeling
talent does what it can
genius does what it must
i do what i get paid to do

#3 doxorubicin

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Posted 06 June 2013 - 02:42 AM

This seems appropriate:
http://www.sciencema.../6042/642.short

#4 doxorubicin

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Posted 04 July 2013 - 06:18 AM

These kits are probably more economical: http://www.markergene.com/oliglo2.php





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