Genomic DNA extraction from filters
Posted 04 June 2013 - 01:21 AM
I am wondering if anyone has experience in extracting prokaryotic genomic DNA (gDNA) from filters using a commercially available kit.
We have started trying to extract gDNA from air samples collected onto filters, using the PowerSoil kit from Mo Bio Laboratories, but have not had much luck; there was no sign of DNA as suggested on the NanoDrop.
Has any of you used modifications of this kit, or have used other kits to acquire gDNA? I have a feeling it is the low amount of microorganisms collected on the filters, but I am new to this project, and would like to just hear about different opinions regarding this issue.
Thank you very much.
Posted 04 June 2013 - 05:26 AM
Posted 04 June 2013 - 02:26 PM
Posted 04 June 2013 - 03:09 PM