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Help! Problem on cDNA library construction


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#1 coral_dai

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Posted 15 March 2004 - 06:11 PM

[COLOR=purple]I met a problem during constructing cDNA library. After blunting the cDNA temini, we resuspended the pellet in 8 l of EcoR I adapters and incubate at 4C for more than 12 hours. Although, the cDNA haven't resuspended, and there is still a big pellet remained. Are there anyone who can help me with this problem? Thank you very much!!!

#2 labrat

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Posted 15 March 2004 - 09:30 PM

Although, the cDNA haven't resuspended, and there is still a big pellet remained.


I am confused :rolleyes: Do you mean "the cDNA have been resuspended, and there is still a big pellet remained." or "the cDNA haven't been precipitated and there is still a big pellet remained."

#3 coral_dai

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Posted 15 March 2004 - 11:51 PM

The second situation. Very little part of it resuspended.




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