ug/vial (protein) 31.9 111.6
units/mg protein (specific activity) 183 113
units / vial 5.84 12.61
per the instructions in the seikagaku batch, i dissolved the solid in 200 ul of 0.1% BSA and made twenty 10 ul aliquots . now im not sure how to reconstitute the sigma batch while maintaing a similar amount of activity in each aliquot!
i ended up calculating the amount of protein / ul and from that determined the amount of ug protein / aliquot, then calculated the units / ug protein to figure out how many units/aliquot. does that sound right? i got ~430 ul 0.1% BSA to dissolve the sigma batch in
my advisor simply saw that there were roughly double the units in the sigma batch but only about 60% of the specific activity and did this calculation
(200ul 0.1% BSA * 2) (0.6) = 240 ul 0.1% BSA
any advise would be appreciated!
Edited by pchu, 03 June 2013 - 01:22 PM.