Primer Design, help i´m New
Posted 03 June 2013 - 08:15 AM
I´m going to tell you my Isuue, you probably going to smile because the solution is very easy :-D
I´m going to do qRt-PCR for GFP; i have a pAc-GFP1-N1 plasmid Vector wich i`m introducing into cells. After an inkubationtime and isolation of total RNA i´m going to do qRT-PCR.
So my problem, in order to do amplification of the AcGFP mRNA, which is translated with an oligT-Primer into c-DNA, i need Primers for the c-DNA.
But i can´´t find the sequence for the AcGFP1-gene of the cDNA. I studied chemistry so my biology knowlege is, not very good:-D
i found programs that calculate the best Primers for ou but i Only have the Sequence of the gene on the plasmid.
What should i do know, because writing down the whol 500 basepairs manually can´t be the right thing to do.
Posted 03 June 2013 - 09:15 AM
Posted 03 June 2013 - 01:21 PM
and i can´t find the sequence of the cDNA or a Program which can konvert the Genesequence into it.
Thank you so much
Posted 03 June 2013 - 02:28 PM
Posted 04 June 2013 - 01:05 AM