Would appreciate your help and suggestions,
FL
Edited by Fluoresce, 01 June 2013 - 10:23 PM.
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Cell Debris Isolation From Blood
Started by Fluoresce, Jun 01 2013 10:22 PM
Cell Debris Blood particles Positive selection
3 replies to this topic
#1
Fluoresce
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Posted 01 June 2013 - 10:22 PM
Does anyone have any suggestions for isolating cell debris/particles from blood without using ultracentrifugation?
Would appreciate your help and suggestions,
FL
Would appreciate your help and suggestions,
FL
#3
gfischer
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Posted 06 June 2013 - 01:42 PM
Have you considered a density gradient medium like iodixanol? Without more details as to what you're trying to accomplish, it's difficult to give much in the way of advice.
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#4
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Posted 08 June 2013 - 11:34 AM
Hi gfischer...
Thanks for your reply. No I was more thinking in the direction of filteration (tandem filteration) using micro filter polymers. But iodixanol seems to be a promising way as well. I need to be able to separet the blood particles from stressed blood and study them in a microfluic system (i.e spike certain amount of these debris into intact blood and run them in a microfluidic device to find the threshold for failure). Do you have a good protocol to suggest for using iodixanol for this purpose? I would really be very grateful if you could help me out with this.
Thanks!
FL.
Thanks for your reply. No I was more thinking in the direction of filteration (tandem filteration) using micro filter polymers. But iodixanol seems to be a promising way as well. I need to be able to separet the blood particles from stressed blood and study them in a microfluic system (i.e spike certain amount of these debris into intact blood and run them in a microfluidic device to find the threshold for failure). Do you have a good protocol to suggest for using iodixanol for this purpose? I would really be very grateful if you could help me out with this.
Thanks!
FL.
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