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Protein measurement of Laemmli samples

suggestion?

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2 replies to this topic

#1 Tai

Tai

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Posted 31 May 2013 - 07:27 AM

Hello...

I'm asking about what is quick and reliable protocol/method to measure total protein concentration from human whole cell extraction samples with Laemmli buffer ??

Now I'm using Rc Dc protein assay from BioRad . It works fine but it's quite slow for me ( take time like 4 hours for my 20-30 samples)

does anyone know if which technique that could measure total protein concentration in Laemmli buffer sample? and could finish 20-30 samples in less than hour Posted Image

Thank you in advance for any suggestion!

//Tai

Edited by Tai, 31 May 2013 - 07:33 AM.

I h..ve ne..er finish.. ...ny thi .....

#2 JoeSus

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Posted 01 June 2013 - 03:19 AM

Hi,

The Peirce BCA protein assay is supposed to be quite fast, and after a quick look through the manual I dont think it should Laemelli buffer should interfere, I've put the link underneath. Also, are you using a 96 well plate in a plate photometre? that is such quicker than running it on a Nanodrop.

Joe

http://www.piercenet...?fldID=02020101
http://www.piercenet...ons/2161296.pdf
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#3 Tai

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Posted 02 June 2013 - 09:21 AM

I'm checking ... thank you very much..
I h..ve ne..er finish.. ...ny thi .....




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