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heterologous expression in yeast

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#1 deespike



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Posted 30 May 2013 - 07:14 AM

hi all

i am trying to perform RT-PCR from rna isolated from yeast strain transformed with yeast expression vector under gal1 promoter. I have failed so many times as i am unable to detect any expression by using oligodT primers for c-dna preparation. I harvest the cells after they reach OD <1. i.e after 16-18 hrs.

please help me

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