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Growing certain number of bacterial cells in LB

serial dilution e.colli growth

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#1 Morty

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Posted 30 May 2013 - 05:48 AM

Hi all,

I am trying to grow around 150 E.coli cells in 200 LB. Anyone knows how long it takes to turn into exponential phase?

Thanks

#2 bob1

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Posted 30 May 2013 - 02:02 PM

200 what - ul, ml, l...?

The time taken will depend on the strain, the age of the colony picked, the temperature and aeration.





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