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how to detect the modification like phosphorylation on one amino acid locus of o


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#1 savetld

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Posted 29 May 2013 - 10:27 PM

I have one mutation on one protein. I would like to know if the modification on this mutated amino acid is different from wild type. Any method is capable to detect it? Thx.

#2 mdfenko

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Posted 30 May 2013 - 06:30 AM

here are some of which i am aware:

depending on the modification and site

phosphorylation can be detected by western blot or elisa (if the epitope is exposed) with an antibody to the phosphorylated site.

methylation can be detected by microarray and sequencing.

glycosylation can be detected by mass spectrometry.

there are most likely other ways.

Edited by mdfenko, 30 May 2013 - 06:32 AM.

talent does what it can
genius does what it must
i do what i get paid to do

#3 mdfenko

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Posted 31 May 2013 - 03:26 AM

oops, the methods for detecting methylation are for dna (sorry).
talent does what it can
genius does what it must
i do what i get paid to do




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