Posted 29 May 2013 - 05:03 AM
In my view, overlaping peaks may be due to the problem with sequencing itself or it might even be genuine due to an heterozygous deletion. these you can predict when you look at your chromatogram; if the overlapin is right frm the start and the peaks doesnt look good then it mostly due to seq errors casued by impure/less sample or non-specific product or even bad sequencing. but when the overlapin starts in the mid suddenly, until then the single peak looked good, then it might be due to one of the allele has got a base/bases deleted and so the overlaping occurs with the successive base peaks. just think abt this and analyse ur sequence you 'll find an answer.
I would prefer being perfectionist rather than a passionist in Research.
I always had an alternate hypothesis....