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Primary antibody


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#1 Suhaila Taher

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Posted 27 May 2013 - 11:55 PM

Hey guys,

I left my primary on shaking in the fridge for a week. Just remembered today! It has been shaking in a glad wrapped dish for the whole week. Is it alright to probe for secondary or would there be a lot of nonspecific binding since?

Sue

#2 Curtis

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Posted 28 May 2013 - 02:26 AM

just go ahead, but there will thick background. you must wash well before adding secondary.




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