I have been assessing the expression of Angiotensin II, AT1 receptor proteins in rat synovial tissue. When analysing my gels, i find that there are two bands in the region of interest, situated very close to each other. I realise this could be due to phosphorylation or oxidation, however i am unsure of how to get rid of this problem.
Is this a serious problem that could prevent publication?
How could i assess whether or not this is due to phosphorylation or oxidisation?
I have attached a recent picture...i have cleaner ones, but not on this computer!
Edited by Limp_chimp, 12 March 2004 - 01:09 AM.