Less than 10 GFP positive cells
Posted 26 May 2013 - 01:55 PM
I harvested the tissues (such as pancreas, liver and spleen) in order to detect if there is any GFP+ cells
Is it possible to analyse/sort the tissues samples using FACS to quantify the number of GFP+ cells which might be less than 10 cells!?!
Posted 25 June 2013 - 11:18 AM
just make sure you have a negative control, no GFP expressing cells. If your wanting to look at cells from transgenic mice, get a sample from a mouse that is not supposed to express GFP. If this is from transfected cells, set aside a aliquot of cells that were not transfected. These controls are vital to determining from where your GFP signal is coming. All cells have some level of background fluorescence and this differs between cell lines and cell types... so make sure the cell types are the same.
Edited by rdvelvet02, 25 June 2013 - 11:19 AM.