Sensitivity of RT-PCR and qPCR
Posted 26 May 2013 - 01:50 PM
I inject GFP stem cells into a normal mouse and i harvested the tissues (such as pancreas, liver and spleen) in order to detect if the GFP+ cells are successfully transplanted/home into the mouse.
Is it possible for me to do RT-PCR or qPCR to detect the level of GFP+ cells? Which is more sensitive because maybe only 1 or 2 GFP+cells in whole tissues..Or any other technique besides immunohistochemistry?
I have no idea if the GFP+ cells are transplanted successfully into the mouse by the way
Posted 27 May 2013 - 01:55 PM
Posted 29 May 2013 - 08:45 AM
Thanks alot for the suggestion but I am confuse, how do i take the harvested tissues and spike them on the normal tissues/cells?
Posted 29 May 2013 - 03:18 PM