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are they non-specific band?


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4 replies to this topic

#1 man121

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Posted 25 May 2013 - 10:25 PM

I am student and I did a PCR. The expected band size is 181bp. And I found that there is a fade band below my expected band. Are they non-specific bands? or degradation? Thx

PCR.jpg

#2 pcrman

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Posted 25 May 2013 - 11:17 PM

The lower bands appear to me to be specific bands, they could be an isoform of the gene. What kind of PCR (gDNA or cDNA) are you doing?

#3 man121

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Posted 26 May 2013 - 12:29 AM

It is gDNA, how could I avoid it?

#4 bob1

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Posted 26 May 2013 - 01:04 AM

So what have you done to optimise the reaction?

#5 JoeSus

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Posted 04 June 2013 - 03:23 PM

It might be worth checking the melting plots to confirm its a specific band; there will likely be a second smaller peak near the main peak.




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