Posted 24 May 2013 - 08:36 PM
Posted 26 May 2013 - 11:57 PM
Just follow the paper which you are citing right now.
if some problems reveal. make a modest modification, then.
Posted 27 May 2013 - 04:47 AM
Posted 28 May 2013 - 05:13 AM
mobility in o-d gels may be low (won't be useful for size determination) but should still be in the correct direction. also, most of the proteins which may be along with your protein will have a pI<7 so it may still be a better choice than acid page.
either way, you'll want to use a 12 or 15% gel.
genius does what it must
i do what i get paid to do