removal of ethanol in PCR product purification
Posted 24 May 2013 - 07:56 AM
Now how can i remove this ethanol from the elute ??? After this step i placed elute at 4C.
Please give me some ideas to remove ethanol
thnax in advance
Posted 24 May 2013 - 08:46 AM
Posted 26 May 2013 - 01:10 AM
Thanx ashu, but what about he DNA in it?
What temp. is your body at?
Posted 26 May 2013 - 05:50 AM
To be completely sure (albeit with the risk of losing some amount of DNA) I would precipitate the eluate with like 75 ul of 100% ethanol, leave overnight at -20 and then centrifuge at 12 000g/10 min/4 degrees and discard supernatant, briefly dry and then dissolve in what amount of water or buffer you want (but note you probably won't see any pelet, but unless it's a really low DNA concentration, it would be there, I use this protocol for desalting PCR products after restriction).
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