Posted 22 May 2013 - 11:18 PM
I'm studying the possible delivery of one particle into the mitochondria. Using confocal microscopy and Mitotracker I colocalize my particles and mitochondria, but it still doesn't mean that particle is entering the mitochondria and ending up in the lumen.
What kind of microscop could ou recommend me to distinguished between outer and inner membrane and lumen?
I has tu be super resolution microscopy, do you thing I can do it using cryo-tem or TEM or some other propositions?
Posted 23 May 2013 - 12:48 AM
This is a suggestion: there is a method for further fractionation of mitochondria to separate inner, outer membrane and the matrix, using sucrose gradient, if I'm not mistaken. You need to google. I mean after you do subcellular fractionation, you can further fractionate mitochondria, to find where your particle is located. no need for TEM. This is faster.
- Angelina007 likes this
Posted 26 May 2013 - 07:26 AM
I'm using polimer of natural polysaccharide of size 2-7 nm.
Probably by now I can't send pictures (going to be published in other paper)