Stain in electrophoresis is going from + to -
Posted 22 May 2013 - 09:48 AM
I am using Serva Dna Stain G. So I have to run gel quite long because I have 2 framgents (one is much bigger than the other one) but when I am running a gel (1%) after 45 min at 75V the stain is going up (like almost to half of it) so then I can't see any bands below. So I the stain is negativly charged.
As I remember with EtBr I have not such a problems? Or my memory is messing?
So the only think that I can try is post-staning? Or there is something else?
of course I can also do a extremely long gel
Posted 22 May 2013 - 12:44 PM
Posted 22 May 2013 - 02:31 PM
Just for curious with EtBr is the same problem?
and how good is post staining?
Posted 22 May 2013 - 03:44 PM