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Designing cloning primers for DNMT


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#1 pmggms2

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Posted 22 May 2013 - 06:48 AM

Good Morning,

I am having a little a little bit of trouble with designing methyltransferase primers. I have conducted a blastp and obtained my sequence of interest, but I am confused as to what I should do as far as creating my primers by using the CDS (coding sequence) or the whole genomic sequence. In order to clone the full length DNMT. PLEASE HELP!!!!!

THANK YOU in advancePosted Image Posted Image Posted Image Posted Image

#2 pcrman

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Posted 22 May 2013 - 07:25 AM

Which organism and which DNMT? There are several DNMT genes. please specify.

#3 pmggms2

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Posted 23 May 2013 - 07:34 AM

So far I have found CMT's, MET's and DRM's in phaseolus using bioinformatic tools.




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