Designing cloning primers for DNMT
Posted 22 May 2013 - 06:48 AM
I am having a little a little bit of trouble with designing methyltransferase primers. I have conducted a blastp and obtained my sequence of interest, but I am confused as to what I should do as far as creating my primers by using the CDS (coding sequence) or the whole genomic sequence. In order to clone the full length DNMT. PLEASE HELP!!!!!
THANK YOU in advance
Posted 22 May 2013 - 07:25 AM
Posted 23 May 2013 - 07:34 AM