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Yeast complete cDNA library

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#1 pratimasaini



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Posted 22 May 2013 - 04:55 AM

I am using bait in pGBKT7 and cDNA library in pGADT7 and using the clonetech's protocol for libarary screening. I have selected the blue colonies from sd/-trp/-leu/-his/-ade/ x alpha gal.
When I retreak these colonies on sd/-trp/-leu/-his/-ade, these clones grow. Wen i try to confirm it by pcr, no band appear neither from yeast plasmid nor from colony pcr.
indly sugest me any solution.

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