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Extraction of Transmembrane Proteins


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#1 criscastells

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Posted 21 May 2013 - 07:51 AM

Hi everybody,

I will start to analyze E-cadherin by Western Blot. I am not sure about the extraction methodology. There is any special issue for the extraction of Transmembrane Proteins or can I use RIPA buffer as usual?

Has someone any special tip?

Thanks you very much in advance!

#2 GNANA

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Posted 21 May 2013 - 08:19 AM

RIPA shd be fine.
I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....




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