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cell seeded 3D collagen 1 gel

protocol cellseeded collagen 1 collagenase 1 3D collagen gel

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Posted 21 May 2013 - 04:04 AM

hi, every one i am new to this forum and really impressed with the work and help you all guys put in.

i desperately need a protocol to set up a 3D model of cell seeded in collagen 1 gel, specially for a thicker layer of collagen
i am using human osteosarcoma ,Mg63 cell line,
also want to mount the gel on glassbottom 30mm petridish for confocal imaging,i failed to adher the collagen gel to the glassbottom, it always constricts, hope you guys can guide me.

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