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which to use: miR or miRexpression vector

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#1 mahrak



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Posted 21 May 2013 - 03:20 AM

hi everybody
I am going to transfect SW480 cell line with miR143 in order to prove the concept of "gain of function". and I was wondering if anybody could help me to make the dicission of using miR-143(as in oligonucleotide miR-143) or miRexpressin vectors.

any response it much appreciated in advance.

PS: developing a stable cell line is not an option

#2 pcrman



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Posted 21 May 2013 - 10:59 PM

If stable miR expressing line is not an option, then mimics transfection can be the first choice, although stable precursor miRNA expression represents a more natural way of overexpressing a miRNA. Ideally, it is great to have both mimics transfection and stable expression.

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