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Genomic DNA extraction


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#1 eyore

eyore

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Posted 08 March 2004 - 12:15 AM

Does anyone has a good method to extract genomic DNA from gram positive bacteria? I now use lysozyme at 37C for 1 h then add 10%SDS and incubate at 65C until solution is clear and after phenol:chloroform is addded then mix slowly by using cutting pipette tip and swirl gently. But I can not obtain a good one after check by agarose gel. Thanks.




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