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[SF_HK]

Hi,

I plan to do a colony foration assay (or foci formation assay) to observe the effect of my plant extract, of certain concentration,  on cancer cell lines.  I plan to seed about 300-500 cells per 60mm dish and observe the no. of colonies that form after about 2 weeks.  In these two weeks,  can I change medium (containing the right dosage of the extract)?

[bob1]

You can change the medium as this would be similar to giving repeated doses as a therapy, though it depends if you want this to be the case or not.